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Both teams remain level on points with 36 each, with Juventus holding a slight advantage on goal difference. They sit in fourth place, one point behind third-placed Napoli, three behind leaders Inter, and two behind second-placed Milan.
Inter and Napoli play Parma and Verona respectively on Wednesday, while Milan face Genoa on Thursday.
Luciano Spalletti's team entered the match on the back of a 1-1 draw at home against Lecce in the previous round, following a run of four consecutive victories both domestically and in Europe. However, they returned to winning ways on Tuesday after taking the lead thanks to an own goal by Bosnian Tarik Muharimovic, who deflected a cross from Frenchman Pierre Kalulu into his own net (16').
The score remained unchanged until halftime, then Juventus sealed the victory with two goals in less than a minute. Fabio Meretti opened the scoring in the 62nd minute, assisted by Canadian Jonathan David, who then added the third himself following a swift counter-attack (63').
Roma, for its part, entered its match against Lecce on the heels of a loss to Atalanta, the former club of its new coach Gian Piero Gasperini.
The Giallorossi, who were top of the table before their 2-0 defeat to Napoli in the 13th round, now lead Como by three points after the latter's 3-0 victory away to Pisa on Tuesday.
Roma finished the first half with a 1-0 lead thanks to a goal by Irishman Evan Ferguson in the 14th minute, following a well-worked team move and a pass from Argentinian Paulo Dybala.
The capital club effectively secured the three points in the 71st minute after a corner kick taken by Dybala found Nicolo Pizzelli, whose shot was deflected into the path of Ukrainian Artem Dovbek, who scored the visitors' second goal.
However, Dovbek's joy was short-lived, as he was forced off the field injured four minutes before the end of regulation time.
Como achieved their third consecutive win and ninth of the season with goals from Argentinian Maximo Perrone (68') and Greek Anastasios Dovkas (76' and 90+6' penalty). Pisa missed a penalty in the 85th minute, taken by Angolan Mbala Nzola.
Cesc Fabregas' team moved up to fifth place with 33 points, three points behind Juventus and Roma, with a game in hand against Milan on March 15.
Meanwhile, Pisa remain rooted to the bottom of the table with 12 points, level with 18th-placed Verona and 19th-placed Fiorentina, and three points adrift of safety.